OUR WORKSHOP

We developed an end-to-end experimental pipeline allowing quantification of In vivo fluxes in photosynthesis and central carbon metabolism from cell to mass-spec. This pipeline employs cutting edge metabolomics, microfluidics and phenotyping systems and is constantly improved to meet sensitivity and throughput requirements, and to integrate with new computational tools. 

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 CO2 labeling setups To support isotopically non-stationary metabolic flux analysis (INST-MFA), we developed a set of tailor-made metabolic-phenotyping setups based on combining gas mixers with microfluidics for liquid cultures (algae, cyanobacteria) or gas chambers for higher plants. Label is supplied via medium pre-bubbled with   CO2-based synthetic air mixture (algae) or with the air mixture itself (plants). 

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Photosynthesis-focused Metabolomics The analytical infrastructure in the lab includes metabolomics analysis of most photosynthetic intermediates, including the CBC, sucrose and starch synthesis, photorespiration, C4 photosynthesis, and additional downstream central C metabolism pathways. Focusing mostly on polar metabolites, our main setup is a bio-compatible uHPLC system linked to a high-end tandem MS (Sciex Qtrap 6500+) equipped with Ion Mobility technology (SelexION).

A major effort is currently dedicated to implementing new separation techniques (HILIC, Anion-exchange) which will bypass the need for ion-pairing reagents involved in the separation of phosphorylated intermediates of photosynthesis, in order to extend the pool of studied metabolites and analytical applications.

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Photobioreactors Our basic experimental setups for growth and physiology analyses, are state-of-the-art photobioreactors which allow full and accurate control and monitoring of algal growth over a wide range of conditions. Following the development of our microfluidic setups, we are currently designing the next generation of this system, which will allow high-throughput pulse-labeling via an automated and controlled auto-sampler. If you’re an enthusiastic experimentalist with a crave for an engineering challenge, let’s talk